In order to screen probiotics that can inhibit the growth of Streptomycetes spp. that produces geosmin (GSM) and 2-methylisobcamol (2-MIB) in aquaculture, Streptomyces thermocarboxydus, S. cyaneofuscatus and S. roseoflavus were used as test strains to carry out inhibition experiments, and the screened strains were identified. The results showed that eight strains of probiotics had inhibitory effects on the growth of S. spp., 2 strains of them could inhibit the growth of S. thermocarboxydus and S. roseoflavus, meanwhile, the other 6 strains affected the growth of S. thermocarboxydus. 2 strains belonged to Bacillus licheniformis and 6 strains belonged to B. subtilis.The 8 screened strains can serve as probiotics for preventing and controlling off-flavor, providing theoretical reference and practical basis for biological control and removal of off-flavor in aquaculture.