The parental sizes of Chinese mitten crab （Eriocheir sinensis） and the growth performance of its offspring have become interestingly main topics in aquaculture industry in recent years. In this study， we applied individual growth comparison and metabolomics techniques to investigate molting and growth characteristics， intestinal microbes and hepatopancreas metabolites compositions in offspring （Size A， Size B and Size C）of the three parental standards of Chinese mitten crab （200-250 g for females and 300-350 g for males； 250-300 g for females and 350-400 g for males； > 300 g for females and > 400 g for males）. The results showed that the growth performance in the offspring of size C was better than the other two sizes in the two individual molting and growth measurements， but there were no significant differences in the molting weight gain rate， molting interval， fatness， hepatopancreas index and muscle index among the three parental sizes of offspring. The results of intestinal microbes analysis showed that α diversity index of the size A was lower than the other two sizes， but there was no significant differences among them， while the PCoA analysis showed significant differences of intestinal microbes composition among the three sizes at the genus level. Metabolomics analysis of hepatopanacas found a total of 100 different metabolites between the sizes A and B， 77 different metabolites between the sizes B and C， and 79 different metabolites between the sizes A and C. Pearson correlation analysis between different intestinal microbes and metabolites revealed that the metabolite Biopterin was significantly positively correlated with Genus Paraclostridium in the size A； the the metabolite N-acetyl-d-mannosamine was significantly negatively correlated with Phylum Nitrospira and Genus Nitrospira in the size B； the metabolite tetrahydrodipyridinate carboxylate was negatively correlated with Genus Lactobacillus in the size C. The current results of this study indicated some differences have been detected in molting and growth， intestinal microbes and hepatopancreas metabolites composition among the offspring of different parental sizes in Chinese mitten crab.