The omd gene encodes osteomodulin， which regulates mineralization in human bone. However， the effect of omd gene on bone mineralization in fish is still unclear. To explore the effect of omd gene on fish skeleton， we investigated the expression of omd gene in different developmental stages and different tissues of zebrafish. Using CRISPR/Cas9 gene editing technology， we constructed omd gene knockout zebrafish （omd^-/-）. The results showed that the expression of omd gene gradually increased with development in zebrafish， and the expression was higher in vertebral column and muscle. Compared with the wild-type zebrafish，no significant changes were found in bone morphology and tail muscle structure of omd^-/-zebrafish， and the calcium content of the vertebral column was down-regulated by 59.81%. Compared with the wild type， the average swimming distance and swimming speed of omd^-/- zebrafish decreased by 33.93% and 39.44%， respectively， while the relative stationary time increased by 88.26%， and the swimming ability decreased. These results suggest that the deletion of omd gene affects mineralization of the vertebral column of zebrafish and affects the mobility of zebrafish to a certain extent.