In order to tap the potential of high-value utilization of Chlamys nobilis, using compound protease to hydrolyze Chlamys nobilis adductor muscle, the effect of EHCA(enzymatic hydrolysate of Chlamys nobilis adductor muscle) on the activity of α-glucosidase and the scavenging ability of DPPH free radicals was explored.The fasting blood glucose concentration, serum insulin content, liver malondialdehyde and superoxide dismutase content were used as indicators to evaluate the auxiliary hypoglycemic function and antioxidant activity of EHCA in normal mice. The results showed that EHCA had good α-glucosidase inhibitory activity, and its DPPH free radical scavenging ability increased with the concentration of the enzymatic hydrolysate. The results of animal experiments showed that a certain concentration of EHCA could enhance the glucose tolerance of mice, significantly reduced the liver malondialdehyde and increase the activity of superoxide dismutase. In conclusion, the compound protease can effectively enzymolyze the adductor muscle of Chlamys nobilis, and its enzymatic hydrolysate has good α-glucosidase inhibitory activity and antioxidant activity with a certain auxiliary hypoglycemic effect. The results may provide a theoretical basis for the development of auxiliary hypoglycemic marine functional foods.