In order to develop herb compound preparation against Aeromonas veronii pathogenic to Eriocheir sinensis, the present study used A. veronii HXH1 as the indicator strain to screen herbs with good inhibitory activities by tube dilution method, and on this basis, the compatibility of these herbs was optimized by orthogonal t design to make Chinese herbal compound preparation, and the in vitro antibacterial effect of the Chinese herbal compound preparation was further evaluated using tube dilution method. The results indicated that Rhizoma coptidis, Caesalpinia sappan, Radix et rhizoma rhei, Fructus mume, Spatholobi caulis, Punica granatum, and Moslae herba showed highly inhibitory activities against A. veronii, with the minimum inhibitory mass concentrations (MICs) ranging from 7.81 to 31.25 mg/mL against A. veronii HXH1. However, R. et rhizoma rhei, C. sappan and F. mume were found to have negative effects. Thus, R. coptidis, M. herba, C. sappan, P. granatum were chosen as main components of the Chinese herbal compound preparation, and the best compatibility proportion was determined as 5:4:1:2, under which the MIC of the compound preparation was 5.21 mg/mL against A. veronii HXH1. In addition, the Chinese herbal compound preparation exhibited good activities against other A. veronii isolates from E. sinensis, showing the MICs of 3.91 to 7.81 mg/mL against strains HXY1, HXY3, J1Y4, J1Y5, J2Y4, J2Y6, WAB1. This study determined the main components and the best compatibility proportion of herb compound preparation against A. veronii pathogenic to E. sinensis, and can provide a basis for the herb control of diseases in E. sinensis.