To evaluate the effects of dietary proline supplement on growth, body composition and antioxidant capacity of Nibea coibor, a total of 450 Nibea coibor were randomly assigned into six treatments and fed with diets supplemented with different levels of proline (0, 5, 10, 15, 20 and 25 g/kg of dry diet) for eight weeks. After feeding, growth, body composition and antioxidant capacity of Nibea coibor were determined. The result showed that dietary proline levels had no significant effect on the growth of Nibea coibor. However, proline affected significantly the crude protein of whole-body in Nibea coibor. The optimum amount of dietary proline was estimated to be 14.73 g/kg based on the crude protein of whole-body. Increasing proline levels supplementation enhanced significantly glutathione (GSH), glutathione-peroxidase (GSH-PX) and catalase (CAT) in liver and serum of Nibea coibor and superoxide dismutase (SOD) in the serum. Contrary, increasing proline levels supplementation decreased significantly the malondialdehyde (MDA) in Nibea coibor. In conclusion, proline supplementation does not affect growth performance of Nibea coibor. However, it promotes protein synthesis and enhance antioxidant capacity of Nibea coibor.