Black sodier fly larvae meal is a potential high-quality protein source for fish meal replacement in aquaculture, which has attracted much attention in recent years. This experiment, with juvenile Takifugu obscurus with an initial average weight of (16.33±0.34) g, was aimed at evaluating the utilization efficiency of black sodier fly larvae meal in the diet. Four isonitrogenous and isolipid experimental feeds were prepared, with black soldier fly larvae meal as a substitute for fish meal in different proportions(0, 8%, 16% and 24%, designated as HM0, HM8, HM16 and HM24, respectively). Each group was fed to 3 replicate tanks, with 30 fish in each tank. Fish were fed to satiation three times a day. The feeding trial lasted for 56 days. The results showed that the supplementation of black soldier fly larvae meal had no significant effects on the growth, body composition, muscle composition, muscle amino acid composition, muscle texture and serum biochemical indexes of experimental fish. Compared with the control group, the weight gain rate was increased by 19% in group HM16, indicating that the replacement of fish meal with black soldier fly meal at a low level had a potential positive effect on growth performance. Compared with group HM8, HM24 decreased the feed efficiency and tended to increase the HSI and VSI. The high content of C12:0 and C18:2n-6 in black soldier fly larvae meal can meet the energy demand and 18C polyunsaturated fatty acids requirement of fish, which increased the content of C14:0 and n-6 fatty acids in fish. In conclusion, replacement of 24% fish meal in the diet of juvenile Takifugu obscurus by black soldier fly larvae meal did not affect the growth, body composition, muscle texture and health status of obscure puffer. A low replacement level (8%-16%) could have potential positive effects on growth performance and feed utilization of obscure puffer.