β-cyclodextrin was used as the wall material, bergamot essential oil and Litsea cubeba essential oil were used as the core material, β-cyclodextrin-bergamot essential oil and and L. cubeba essential oil micro-capsules Langbergen were prepared by the method of cyclodextrin embedding. Essential oil microcapsules were dispersed as antibacterial agents in a water-absorbing pad and encapsulated to prepare active antibacterial fresh-keeping pads loaded with essential oil microcapsules. The effect of the active fresh-keeping pads on the preservation of grass carp was investigated. The results showed that compared with non-functional absorbent pads, for the absorbent pads packed with β-cyclodextrin-essential oil microcapsules packaged grass carp, total viable counts (TVC), total volatile basic nitrogen (TVBN) pH and thiobarbituric acid(TBA), were significantly lower than that in the blank group (P <0.05), there by reducing the changes in the texture of grass carp meat hardness, elasticity and resilience. It shows that the sustained release of the essential oil in the microcapsules has a positive effect on the preservation of grass carp. Compared with the blank group, under the condition of (4 ±1)℃ cold storage, the active preservation pad of the experimental group can effectively delay the spoilage of grass carp, and can extend the shelf life of grass carp by 2 to 3 days, and the extension time can reach 50% of the original shelf life.