To eliminate the influence of herbicides and heavy metals on the growth stress factors of submerged plants in the process of ecological restoration, glyphosate and copper were selected as targets.The harvested Myriophyllum verticillatum L. was carbonized at 450 ℃ for 2 hours to make biochar (HW450).The effect of HW450 on the adsorption effect of glyphosate, copper and other single pollutants in water under pH value, biochar dosage and other experimental conditions was studied.The interaction between glyphosate and copper in composite polluted water was investigated, and the optimal adsorption conditions of HW450 for glyphosate and copper composite pollution were optimized by response surface method.The results showed that the removal rate of copper and glyphosate increased with time from fast to slow and reached a balance after 48 hours.In the adsorption saturation range of biochar, the removal rate increased with the increase of solution concentration.In a suitable pH range, the removal rate of glyphosate and copper increased first and then decreased, and copper changed significantly compared with glyphosate.Increasing the amount of biochar can improve the removal rate of glyphosate and copper.In compound pollution,copper can promote the adsorption of glyphosate by HW450.The high concentration of glyphosate can inhibit the adsorption of low concentration of Cu²⁺, and the removal rate of high concentration of Cu²⁺ was less affected by the concentration of glyphosate.The response surface showed that the influence of the three factors on the removal rate was ranked as follows: HW450 dosage > Cu²⁺ concentration >pH value. Under the conditions of pH = 5.21, HW450 dosage = 0.06 g, Cu²⁺ concentration = 27.32 mg/L,the optimal adsorption effect of composite pollution can be obtained: the removal rate of glyphosate was 97.44%, and that of Cu²⁺ was 100%.