In order to explore the difference in growth performance and culture effect of Chinese mitten crab inbred families and hybrid families derived from Liaohe River and Yangtze River during adult Eriocheir sinensis culture stage, the juveniles were reared to adult crabs under similar pond conditions. The growth, puberty molting rate and gonadal development were comprehensively compared, and the differences in survival rate, yield, feed coefficient ratio and final body mass distribution were further evaluated.The results illustrated that there was no significant difference in the mean body mass, weight gain rate (WGR) and specific growth rate (SGR) of Eriocheir sinensis during the growth stage (P>0.05).The peak of female puberty molting appeared from July 25 to August 25, and the peak of male puberty molting did from August 25 to September 25. Compared with Eriocheir sinensis from Yangtze River basin, no significant earlier puberty molting was observed from Liaohe River. From September to November,the hepatosomatic index (HSI) of Eriocheir sinensis individuals gradually decreased, and the gonadosomatic index (GSI) showed a significant upward trend. Significant differences were observed in survival rate, yield, feed coefficient ratio among these four inbred and hybrid families (P<0.05).The final body masses of the adult females were concentrated from 100.00 g to 175.00 g,and the males were concentrated from 175.00 g to 225.00 g. In summary,there was no significant difference in growth performance between inbred families and hybrid families derived from Liaohe and Yangtze River basins (P>0.05).After adaptive culture of Eriocheir sinensis from Liaohe River basin, the growth performance was improved, and the two-year early-maturing trait disappeared.The hybrid families with Yangtze River basin of Eriocheir sinensis as the female parent had higher survival rate and yield.