The 60-day-old blades of wild-type strain (PS-WT) and mutant strain (PS-L) of Pyropia suborbiculatain were used as experimental materials to obtain their isolated cells by enzymatic hydrolysis, and then observe the development and morphology of isolated cells in submerged culture. Meanwhile, we also investigated the effects of temperature and light intensity on the development and differentiation of isolated cells. The results showed that the isolated cells could develop into different types of regenerative plants: normal blades, abnormal blades, cell-masses and sexual cell-masses. In the range of 10-80 μmol photons/(m²·s), there was no significant difference in the percentage of normal blades, cell-masses and conchocelis of the two strains; appropriate light intensity [40-60 μmol photons/(m²·s)] could promote the release of monospores of regenerative plants. In range of 18-24 ℃, the proportion of normal blades and abnormal blades of two strains had no significant difference. However, high temperature (27-30 ℃) could inhibit the formation of normal blades, promote the formation of abnormal blades and the release of monospores the of regenerative plants.