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首页 > 过刊浏览>2019年第28卷第5期 >765-771. DOI:10.12024/jsou.20190302544
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饥饿胁迫对团头鲂鳃组织结构及Na+/K+-ATP酶、抗氧化酶的影响
作者:
基金项目:

国家科技支撑计划（2012BAD26B00）；国家自然科学基金（31572220）；上海高校知识服务平台（ZF1206）；上海地方高校能力建设项目（18050501900）


Effects of hunger stress on the gill microstructure and Na+/K+-ATPase, antioxidant enzyme of blunt snout bream Megalobrama amblycephala
Author:
  • WANG Dongdong

    WANG Dongdong

    Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
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  • WU Chengbin

    WU Chengbin

    Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
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  • ZHENG Guodong

    ZHENG Guodong

    Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
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  • CHEN Jie

    CHEN Jie

    Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
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  • ZOU Shuming

    ZOU Shuming

    Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
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  • 摘要
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  • 参考文献 [33]
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    摘要:

    为了研究不同饥饿时间（0、5、10、15、20、25和30 d）对团头鲂耐低氧F4幼鱼鳃组织的影响，在温度（25±1.0）℃、溶解氧（7.0±0.5） mg/L条件下，以体质量为（30.5±2.6） g的团头鲂耐低氧F4幼鱼为研究对象，利用组织切片、扫描电镜技术和分光光度计法研究饥饿胁迫对其鳃组织结构、Na+/K+-ATP酶和抗氧化酶的影响。酶活性结果显示：随着饥饿时间的延长，团头鲂耐低氧F4鳃组织的Na+/K+-ATP、SOD和CAT酶活性逐渐降低（P<0.05），恢复投喂7 d后基本恢复到正常水平。形态学观察显示：随着饥饿时间延长，团头鲂耐低氧F4鳃小片平均伸出长度增加（P<0.05），层间基质的厚度减小（P<0.05），这种变化导致鳃小片呼吸面积增加和层间基质体积减小（P<0.05）。恢复投喂7 d后，基本恢复到正常投喂时的形态。由此可见，在饥饿胁迫情况下，团头鲂耐低氧F4幼鱼抗氧化系统受到严重干扰，鱼体主动调整鳃小片呼吸面积适应饥饿胁迫。

    Abstract:

    In order to research the effects of different hunger time(duration 0, 5, 10, 15, 20, 25 and 30 d)on gills of hypoxia-tolerant F4 generation of blunt snout bream, the gill microstructure, Na+/K+-ATPase and antioxidant enzyme of hypoxia-tolerant F4 generation of blunt snout bream were studied by light, scanning electron microscopy and absorptiometry at (25±1.0)℃ and dissolved oxygen(7.0±0.5)mg/L.The results showed that with the increase of the hunger time, the activity of Na+/K+-ATP, SOD and CAT in the hypoxia-tolerant F4 generation of blunt snout bream was gradually decreased and then restored to normal level after 7 days of re-feeding.Gill microstructure observation showed that the protruding lamella lengths were enlarged, which caused the expansion of the lamella surface area, and the height and volume of the ILCM decreased.After 7 days of re-feeding recovery, the changes in gill morphology were reversed.These results indicated that the hunger stress could seriously disturb the antioxidant system, but fish showed the capacity to alter respiratory surface area in response to hunger.

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引用本文

王东东,吴成宾,郑国栋,陈杰,邹曙明.饥饿胁迫对团头鲂鳃组织结构及Na+/K+-ATP酶、抗氧化酶的影响[J].上海海洋大学学报,2019,28(5):765-771.
WANG Dongdong, WU Chengbin, ZHENG Guodong, CHEN Jie, ZOU Shuming. Effects of hunger stress on the gill microstructure and Na+/K+-ATPase, antioxidant enzyme of blunt snout bream Megalobrama amblycephala[J]. Journal of Shanghai Ocean University,2019,28(5):765-771.

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  • 收稿日期:2019-03-01
  • 最后修改日期:2019-04-30
  • 录用日期:2019-05-22
  • 在线发布日期: 2019-10-15
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