As a short life cycle species, the resources of neon flying squid in northwestern Pacific Ocean fluctuate greatly during the years, mainly because the population is extremely sensitive to mesoscale climate change and changes of environmental conditions of its spawning ground. Therefore, it is particularly important to study the environmental conditions of the spawning ground of neon flying squid and obtain the most suitable temporal and spatial environmental factors. In a stable mesoscale climate model, based on the scientific conclusion that the ratio of the optimal temperature range of the squid spawning field to its spawning range (P_S) is positively correlated with its resource replenishment, this study intends to conduct a regression analysis of the CPUE of the neon flying squid in 1996-2007 and the Ps of 6 468 hypothetical spawning grounds in each month of January-April, screening out the spawning season, spawning grounds and the optimum SST range of spawning grounds which were statistically significantly correlated with CPUE and P_S. Studies indicate:during 1996-2007, the spawning season is from Jan. to Mar.; The optimal spawning range is 136.5°E-167°E, 20.5°N-27.5°N; The optimum SST range for spawning ground is 22.5-26℃. The results of this study contribute to the understanding of the life cycle process of squid and the prediction of its resource supplement, which provides a basis for the sustainable development and scientific management of squid resources.