Molecular markers related with spawning traits in shrimps is limited. At present study, vitellogenin (VG) gene of Litopenaeus vannamei was selected for SNP (Single Nucleotide Polymorphism) marker screening on spawning traits (ie. spawn or not & egg number). Direct sequencing of VG gene was used for SNP loci screening. The detected SNP loci were further correlated with spawning traits. The experiment was based on 150 ablated female shrimps and the four domains of VT gene. During the discovered 37 SNP loci, there were 3 missense mutation, 8 synonymous mutation and 26 nonsense mutation. Further analysis found 4 potential SNP loci whose polymorphism information content (PIC) were between 0.31-0.36 indicated their middle standard of PIC. Single-factor ANOVA analysis was used for the reproductive variables (number of spawns, total egg number, average egg number, and egg number per body weight). Only the locus 3 839 showed the significance difference on total egg number and egg number per body weight (P < 0.05), no significance differences were found in the other 3 potential loci.