Aspiorhynchus laticeps belongs to Osteichyes, Cyprinformes, Aspiorhynchus Kessler, and was mylopharyngondon piceus. A. laticeps was national level to protect animals, so it had very important economic and scientific values. Because 2-phenoxyethanol was easy preparation, low price and rapid action, it acts as a general anaesthetics in fish. This paper regarded anaesthetics concentration(50, 100, 200, 400, 600, 800, 1 000 μL/L), exposure duration(2, 4, 8, 12, 16, 32 min) and dissolved oxygen concentration(4, 8, 12, 16, 18, 22 mg/L) as reference indices, and the effects of 2-phenoxyethanol were focused on as anaesthetics on juvenile A. laticeps(9.91±0.31g). Result showed that induction time and anaesthetics concentration were negative correlation, however recovery time and anaesthetics concentration were positive correlation. Respiratory rate of A. laticeps would decrease when concentration under 200 μL/L in initial period, but would be 11times/10s at mid-late period. Opercular movement frequency decreased with increasing doses of anaesthetics when concentration was equal or greater than 400 μL/L, and at last. juvenile A. laticep would cease respiration. Juvenile A. laticep could be anaesthetized in less than 3 min, with recovery in less than 5 min at 800 and 1 000 μL/L, so effective anaesthesia range was from 800 to 1 000 μL/L. In high concentrations, recovery time decreased with increasing exposure time. Juvenile A. laticep could recover in (100.51±10.60) seconds(P<0.05. Because of different dissolved oxygen concentration, there were differences between recover time:12 mg/L would recover in less than (129±36.50) seconds, which was lowest. 22 mg/L would recover in (406.33±53.20) seconds, which was highest. The highest group was 3.15 times higher than the lowest group (P<0.05).