This study tries to gradually reduce the addition of carbon source to zero,and to explore the effect of domesticating nitrifying bio-flocs on water quality,bacterial community dynamics of bio-floc and the growth performance of shrimp in high-intensive culture system. Experiment was carried out in three runway aquaculture systems at stocking density of 685 shrimp/m³.Water quality results showed that daily addition of glucose at 100%-150% of feed,which can decrease the concentration of TAN,but the concentration of NO₂^--N was not decreased obviously.When the addition of glucose dropped to zero,TAN can be maintained at lower level,and the NO₂^--N concentration decreased significantly.The bacterial community structure was analyzed by using high throughput sequencing technology.At the phylum level,experiment results showed that both the main advantage microflora of heterotrophic bioflocs and nitrifying bioflocs are Proteobacteria and Bacteroidetes.At the class level,dominant microflora of heterotrophic biofloc is Alphaproteobacteria,and dominant microflora of nitrifying bioflocs are Alphaproteobacteria,Flavobacteria and Gammaproteobacteria.After 65 d,study found that without adding carbon source,nitrifying bio-flocs can limit the abundance of heterotrophic bacteria,nitrospira was rising,and developing into dominant bacteria.At the end of the experiment, glucose which is 41.03%±7.86% of feed was added throughout the culture period.The survival rate and harvest of Litopenaeus vannamei respectively were 43.35%±7.57% and (3.03±0.59) kg/m³.The domestication of nitrifying bio-flocs not only saved cost, but also effectively optimized the structure of bacterial communities, and contributed to better water environment which results in the higher growth and survival of shrimp.