Abstract:In order to understand the effect of domestic fish meal with exogenous additives replacing the imported fish meal on China soft-shelled turtle (Trionyx sinensis), six groups of soft pellet feeds were designed and formulated with 67% of imported fish meal group(IFM), 34% of domestic fishmeal +32% imported fish meal group (DFM+IFM), 67% of domestic fish meal group (DFM), 67% of domestic fishmeal based group added 0.2% yolk antibody Group (DFM-Y), 0.05% sodium butyrate (DFM-S) group and 0.05% Bacillus subtilis group, (DFM-B), respectively. Relative weight gain rate and survival rate of Chinese soft shelled turtle(Trionyx sinensis) were calculated at 15 d, 30 d and 60d respectively. Meanwhile, in feeding behavior test, the above feeds were individually marked with one of the rare earth oxides (La2O3,Dy2O3,Sm2O3,Nd2O3,Yb2O3 and Y2O3) at 100 mg/kg, and the feeding preference was measured by the concentration of rare earth elements in feces and in the feed determined with inductively coupled plasma atomic emission spectrometry. Results showed that, 6 different diets did not affect the survival rate of Chinese soft shelled turtle; the growth of the turtle fed with the diet containing Bacillus subtilis could be significantly better than DFM group in 60-day feeding period, even not significantly different for IFM group in 30-day feeding period, while with yolk antibody and sodium butyrate enhancing growth performance only in the early feeding stage(15 d) of the juvenile soft-shelled turtle; feed containing mixture of domestic fish meal and imported fish meal was easier to attract juvenile soft-shelled turtle ingestion. There was no improved feeding effect of egg yolk antibodies, sodium butyrate and Bacillus subtilis in domestic fish meal. There was also no corresponding relationship between initial feeding preference and the growth of juvenile turtle, illustrating the feed ingestion was not the determinant for growth.