In recent years, China's turbot farming industry has been trapped in the mire of oversupply, quality safety and price falling. The regional brand construction may be an important approach to solve these problems. Regional brand construction should be based on a certain extent of industrial agglomeration. This paper therefore uses those criteria of location quotient and industrial concentration to estimate the agglomeration level of turbot farming industry in China, and constructs a theoretical model on the basis of industrial agglomeration, then takes the "Xingcheng turbot" brand as an example to verify the conclusion of the model. The results show that the industry agglomeration of turbot farming industry in China is mainly concentrated in Liaoning, Shandong and Hebei Provinces, and industrial concentration in Xingcheng city is most obvious. The resources, product, market and technological advantages, industry policy guidance and the services of industrial organizations can promote the construction of the brand. But this brand construction process also faces many problems, such as the lack of technical leading enterprise, the lack of overall strategy of the regional brand planning, the existence of "free rider" and "prisoner's dilemma". It is hereby recommended that leading enterprises be encouraged to organize medium and small turbot farms to work closely, establish fish farmers' associations and let the association be the owners of brands. It is also strongly suggested to establish a brand marketing fund and encourage brand-owning farmers to jointly fund advertising and marketing.