In order to study the mechanical properties of tuna longline hooks, and to verify the accuracy of the finite element analysis method, this study conducted tensile test and ANSYS simulation on the ring hook (Type:3.4-4.5). The results of tensile test and ANSYS simulation were compared and tested by ANOVA. The results showed that: (1) there were no significant differences of the mechanical characteristics e.g. displacement or strain, measured by two methods (P>0.05). The maximum strain area was around the back bent and rear side. The maximum total displacement area was around the front bent and front axle. The maximum displacement in X direction was around the bottom and rear side. The maximum displacement in Y direction was around the back bend and back axle; (2) when the tension was 500, 800, and 1200N, the ring hook's strain was 0.002 76, 0.008 14, and 0.069 94; the displacement in X direction was 1.8, 3.5, and 7.2 mm; the displacement in Y direction was -2.4, -4.6, and -8.7 mm; the total displacement was 2.8, 5.4, and 10.6 mm, respectively; (3) there was a significant linear relationship between the results of tensile test and those of the ANSYS simulation. The displacement in total, X, Y direction, and strain of tensile test were 1.018 9, 1.027 2, 1.019 5, and 1.088 3 times the ANSYS simulation. This study suggested that the ANSYS finite element could be used to analyze tuna longline hooks' mechanical property.