Sewage purification by microalgae coupled with microalgal biodiesel production is the highlight of the microalgae technology. This paper studied the growth and lipid characteristics of a high ammonia tolerant microalgae, Chlorococcum sp.,which was cultured at 20,25,30,35 and 40 ℃ respectively for 7 days. The initial inoculation density was 300×10⁴ cells/mL. The result showed that the growth performance of Chlorococcum sp. was affected significantly by culture temperature. The cell density and biomass first increased then decreased along with the increasing temperature. The peaks of cell density and biomass were 3 604×10⁴ cells/mL and 0.92 g/L respectively, which appeared at 35 ℃.The analysis on lipid characteristics of the biomass revealed that the lipid content and lipid yield also first increased, then decreased along with the increasing temperature. The peaks of lipid content and lipid yield appeared at 35 ℃ and were 23.67% and 21.65 mg/L respectively. The saturated fatty acid content in microalgae first increased, then decreased while the poly-unsaturated fatty acid content always decreased along with the increasing temperature. It is therefore suggested that 30-35 ℃ is the suitable culture temperature for Chlorococcum sp.cultivation.