To investigate heterosis and combining ability of the diallel crosses of different Apostichopus japonicas populations which were from Changdao(C), Dalian(D), Weihai(W) and Jiaonan(J), the experiment established 16 groups of 4×4 diallel crosses of different Apostichopus japonicas populations. The larval survival growth performance of 30 day(d), 60 d, 80 d and 105 d were obtained, and the heterosis, the general combining ability and the specific combining ability were compared between the different groups. The results showed that:The body length was different in different groups, and there was no significance between hybrid and selfing groups of 30 d, 60 d and 80 d. The body length of the hybrid groups which were groups of D(♀)×J(♂)(DJ), WD, JD and JC of 105 d was significantly greater than the combinations which were groups of CC, WW and JJ. The heterosis was different in different hybrid groups and different stages. The groups of CD, JC and JD were always positive, and the group of CW was always negative. The number of groups which were positive increased with growth of A. japonicas, and the groups of JC of 105 d was 9.69% and highest. The general combining ability of D(♀), J(♀) and D(♂) was higher than others. The special combining ability of groups of JD was higher on 30 d and 60 d, the groups of CD, DJ, JC and JD were higher than other groups on 80 d and 105 d. It can be concluded that the hybrid combinations of C(♀)×D(♂), D(♀)×J(♂), J(♀)×C(♂) and J(♀)×D(♂) of Apostichopus japonicas have better growth performance and higher special combining ability by comprehensive consideration.