Stable isotope analysis is widely used in trophic ecology study and has been a powerful tool to examine structure and trophic interactions of aquatic food webs. Multiple tissues of organism may provide diet information of their life history. Applications to reconstruction of diet and migration patterns require precise estimates of turnover rate and discrimination factor of each isotope in the studied tissues. The study derived turnover rates and discrimination of yellow catfish Pelteobagrus fulvidraco from the tissues of different organs and estimated the contributions of growth and metabolism through laboratory diet-shift experiment. Results showed that mucus (δ¹³C:28.0d, δ¹⁵N:35.7d) and liver (δ¹³C:25.8 d, δ¹⁵N:63.6d) are the tissues with the highest turnover rates, while the turnover rate of fin (δ¹³C:106.5 d, δ¹⁵N:64.2d) and muscle (δ¹³C:92.6d) are smaller and gill having the lowest turnover rate and the longest half-life. The carbon and nitrogen stable isotope discrimination factors of tissues are different. Discrimination factors of different tissues are-1.4‰-2.4‰ for nitrogen and -2.1‰-2.0‰ for carbon. The growth rate of Pelteobagrus fulvidracois relatively slow, and the contribution of growth to tissue stable isotope turnover rate is low, however, the metabolic contributions to both carbon and nitrogen turnover rate are higher (>60%) in all tissues except for gill. The variation in turnover rates of multiple tissues may provide complementary information for diet reconstruction at different time scales, and the mucus and liver reflect short-time diet shift, while fin, liver and muscle reflect a rather long-time of dietary information.