The paper detected 17 biochemistry indices of the 39 healthy captive hatching larval green turtles (1.5-2.5 years old), six healthy adult green turtles (10-20 years old) and 3 wild turtles using the Beckman AU5800 and TNI BECKMAN Access 2 to analyze. The results showed that there was large difference between wild and captive ones on aspartate aminotransferase (P=0.002), albumin (P=0.015), alkaline phosphatase (P=0.010), glutamic pyruvic transaminase (P=0.010), etc. The biochemistry indexes presented no difference in captive green turtles between different ages and different sexes except for alkaline phosphatase. Alkaline phosphatase and age showed a negative correlation. Compared with the research results of others' and the experiment's wild green turtles, the indexes of wild individuals had great difference which could not be regarded as the reference for health indicators. This study established blood biochemistry reference values of captive green turtles in cardiac, liver and renal functions. This provides a reference for the wild green turtles' blood biochemistry research and established a basis for the early diagnosis and disease prevention of captive green turtles.