A 45-day feeding test was conducted to investigate the effects of different kinds and levels of dietary starch on liver histology of largemouth bass (LMB) with an initial body weight (23.45±0.08) g. Eight iso-nitrogenous and iso-energetic diets were formulated with four kinds of starch (wheat starch, waxy corn starch, high-amylose corn starch, and cassava starch) at 5% and 10% levels, respectively. At the end of the feeding test, liver samples of test fish were collected. Biochemical analysis and histological observation of the liver tissues were performed. The results showed that the liver of fish, fed with all diets containing 5% starch and diet containing 10% high-amylose corn starch, was found bright red as usual, while the liver of fish, fed with the other diets with 10% starch, turned pale. Meanwhile, compared to fish with diets including 5% starch and 10% high-amylase corn starch, the hepatocyte size, hepatosomatic index, hepatic glycogen content and occurrence rates of the hepatocyte vacuolization and nuclei shifting to the cellular periphery were much higher in fish fed other 10% starch diets. It would be concluded that both dietary starch kind and its level can have a significant effect on glycogen content and tissue structure of the liver in LMB, high-amylose corn starch is more suitable than wheat starch, waxy corn starch and cassava starch, and that an inclusion level of 5% dietary starch is more suitable than of 10%.