Effects of agitator speed, aeration rate, inoculation amount and inoculum age on chitosanase production were studied by single factor experiments designs under the conditions of 0.6 filling coefficient and 0.08 Mpa tank pressure. The results showed that fermentation parameters in 10 L tank were defined as following250 r/min agitator speed, 3% inoculation amount, 2.0vvm aeration rate and seed solution of OD₆₀₀=0.8-1.0. Under these conditions, the highest chitosanase activity in fermentation liquid was detected at 52 h during 56 h fermentation period, which reached (35.2±0.5) U/mL. The maximum chitosanase activity was detected at 60 h during a 64 h fermentation period of feed batch fermentation, which reached (46.4±0.5) U/mL. The enzyme production of feed batch process increased 32% by maintaining the mycelial morphology and extending the time of enzyme production stage. This article has made some contribution to industrial production of chitosanase.