The triangle pearl mussel, Hyriopsis cumingii, has uninominal special life cycle, and the glochidia need to undergo a short parasitic life and intake of adequate nutrition from the host fish to ensure that they could complete juvenile metamorphosis. The hydrostatic breathing room method was applied to study effects of infection stress by glochidia of triangle pearl mussel on oxygen consumption rate and ammonia excretion rate in yellow catfish Pelteobagrus fulvidraco, then the survival rate and the appropriate number of glochidia parasitized were analyzed. The results showed survival rate in experimental groups of Pelteobagrus fulvidraco were 100%, 93.3% and 50.0% after the glochidia falling completely; and there was no significant difference in the oxygen consumption rate of the host fish infected by glochidia (P>0.05); the ammonia excretion rate was significantly increased and O/N ratio decreased (P<0.05),and, there was a positive correlation between the quantity of glochidia and ammonia excretion rate, and positive with the mortality of host fish. So it could be concluded that: after being parasitized by the glochidia, a large number of cysts were induced to affect the respiratory system of host fish, thus hindering its use of oxygen.