This paper studied the anesthetic effects of MS-222 at the concentration of 10, 20, 30, 40, 50, 60 and 70 mg/L on largemouth bass(Micropterus salmoides), then it studied the impact of water temperature at 10, 15, 20 and 25 ℃ in 20 mg/L MS-222 on anesthetic effects,and finally anesthesia simulated transportation was conducted at 20 ℃ and 20 mg/L MS-222. Anesthesia test results showed: With the rising of MS-222 concentration, anesthesia time shortened while full recovery time prolong. 10-20 mg/L MS-222 was suitable for largemouth bass anesthesia transportation. Anesthesia time shortened but recovery time increased with decreasing of water temperature. 20-25 ℃ water temperature was suitable for largemouth bass anesthesia transportation. Anesthesia simulated transportation test results showed: The survival rate was 100% after transportation 17h but it dropped to 70% after transportation 24h. Recovery time increased as transport duration increased. Muscle glycogen fell but lactic acid rose after anesthesia transportation. The differences of serum cortisol didn't change significantly but were significantly higher than the initial group (P<0.05). There were no significant differences of aspartate transaminase(AST), urea(UREA) and lactate dehydrogenase(LDH)in the first 10 h transportation, then they all increased significantly (P<0.05). Glucose (GLU) increased with transportation time.