The purpose of this study was to investigate the effect of verapamil on metabolism and elimination of enrofloxacin in ridgetail white prawn, Exopalaemon carinicauda. The shrimps were randomly divided into two groups with different experimental diets. The first group was fed enrofloxacin diet at a dosage of 10 mg/kg·d for seven days and served as control. The second group was fed enrofloxacin diet at a dosage of 10 mg/kg·d for seven days, and simultaneously bathed with verapamil at 4 mg/L for seven days, and as verapamil group. The drug concentrations and elimination parameters were compared. The results showed that the enrofloxcin concentration decreased with the days in hepatopancreas, muscle and gill, and the concentrations of enrofloxacin in tissues were significantly higher in verapamil group than those in control group (P<0.05), however, the concentrations of enrofloxacin metabolite ciprofloxacin in tissues were significantly lower in verapamil group than those of control group (P<0.05). The elimination rate constant (β) and clearance rate (CL) of enrofloxacin and ciprofloxacin were significantly lower in verapamil group than those in control group (P<0.05). The results showed that verapamil inhibited the metabolism and elimination of enrofloxacin in E. carinicauda. Especially, P-glycoprotein might be involved in the elimination of enrofloxacin and ciprofloxacin in E. carinicauda.