Screening a few microbes that can ferment by-product of fish processing into useful nutrients for plant. We selected 44 microbes from fertilizer of microbes, soil and corrupted fish, then we chose microbes by some experiments,such as, single fermented experiment, the experiment of nitrogen fixation, insoluble-potassium solubilization and insoluble-phosphate solubilization simultaneously and collaborative antagonism experiment. Then microbes were identified by morphological observation,physiological and biochemical experiments and building the cluster trees based on 16S rDNA or 18S rDNA sequence data of the strains. Finally, GP2 is Acidovorax, GS4 is Pseudomonas sp., ZP1 is Aspergillus niger, ZP3 is Saccharomyces. GP2, GS4,ZP1,ZP3 can ferment by-product of fish processing into useful nutrients for plant, and they can be used to ferment by-product of fish processing.