During eye development, pax6 and tbx3 define retina field, and rx1 defines optic cup field. We investigated whether pax6, rx1 and tbx3 expressed differently between left and right retinas in Paralichthys olivaceus. Quantity RT-PCR showed that pax6, tbx3and rx1 began to express at 20 hours past fertilization. The whole mount RNA in situ hybridization further showed that pax6 expressed mainly in the left and right retinas, and the expression region of left retina is a little bigger than that in right retina. The signal of tbx3 and rx1 was seen in the retina and their expressional region showed no visible difference between left and right retinas. The difference in expression region of pax6 between left and right retinas reminds us whether there exists a possibility that the difference will cause cell number difference between supraorbital and suborbital areas, which finally causes eye migration through the induction of thyroid hormone during metamorphosis.