In order to estimate the dietary Fe requirement of bluntnose black bream (Megalobrama amblycephala) juvenile, 5 semi-purified diets with different Fe supplementation of 0,120,240,360,480 mg/kg(measured values of Fe contents in 5 diets were 391.40, 492.29, 585.98, 681.85, 776.46 mg/kg,respectively) were formulated with FeSO₄·H₂O as the Fe source. A total of 400 bluntnose black bream with an initial body weight of 50 g were randomly divided into 5 groups with 4 replicates per group and 20 bluntnose black bream per replicate. Each group of fish was fed the diet for 43 days. The results showed as follows: when the Fe supplement amount in the feed was 124.55-130.18 mg/kg(the actual Fe content was 493.26-497.76 mg/kg), bluntnose black bream has the best growth effect. In this experiment,the daily feed intake of bluntnose black bream was 5.10 g/100g body weight /d, so the added Fe content of the feed in the 0.64-0.66 mg/100g weight /d(the total Fe content of the feed was 2.52-2.54 mg/100g weight /d). The survival rate, viscera index, scale weight / body weight, body weight / body length, vertebral bone weight / body weight were not significantly affected by dietary Fe content (P>0.05). But the 0 mg/kg group of vertebral bone length / body length was significantly lower than the 120 mg/kg group (P<0.05). Supplement of Fe is conducive to the growth of the spine of bluntnose black bream.