Genetic diversity of eight Procambarus clarkii stocks in Jiangsu Province (Dapu, Pingwang, Qitang, Guantan, Xishunhe, Taiping, Guchen, Yanghetan) was investigated using 8 microsatellite DNA loci.The result showed that all the eight loci were highly or medium polymorphic.Tests of departures from Hardy-Weinberg equilibrium indicated all 64 loci in 8 stocks were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and most of them have significant heterozygosity deficiency.All of 8 stocks showed higher genetic diversity, and genetic diversity of the stocks in DP was the highest, and that of PW was the lowest.F_ST and AMOVA analysis across all stocks and loci indicated that there was medium level of divergence among 8 stock.F_ST=0.126 (0.05<F_ST<0.15).The UPGMA clustering tree based on genetic distance (D_A) demonstrated that the stocks of PW, QT and DP clustered into one group; TP, XS and GT converged into another group, and GC and YH constituted a branch singularly.