Growth performance of F₂ of 5 specialized strains of Macrobrachium rosenbergii(A,B,C,D and E) was checked by one-way completely randomized design and polyculture in concrete ponds with the fluorescent tag. The results indicated that the ranks of AGR and SGR were D>B>E>C>A in either one-way completely randomized experiment or polyculture in concrete ponds with the fluorescent tag. AGR and SGR by ANOVA and Duncan for strains of multi-average revealed that F₂ of B and D were strains with the best growth traits.Every proportion of body form of F₂ was greater than F₁; Variation coefficient of body weight of F₂ was lower than F₁, the most obvious decline in the comparison of F₂ and F₁ was B, decreasing 49.22%.All of them suggested preliminary benefit of strain selecting.Microsatellite markers were used to analyze the genetic diversity and genetic differentiation of F₂ of 5 specialized strains, including 630 individuals. The results showed the allele number was 6.25; average effective allele number was 3.666 2; average observed heterozygosity value was 0.435 0; the average expected heterozygosity value was 0.707 6. All of these five specialized strains in the ascending order the PIC was C(0.434 0)< A(0.480 4)