To study the anti-inflammatory effect of the extract from visceral organs of Sepiella maindroni (ESM), supercritical carbon dioxide extraction was used to extract the lipid from the visceral organs of Sepiella maindroni (ESM). The acute inflammation and chronic inflammation models were adopted,such as ear-swelling of mice induced by dimethylbenzene,edema of rat paw induced by carrageenin and granuloma induced by implanting cotton ball. The effects of various doses of the lipid extract on the inflammatory rat models were compared with those of the blank control and the positive control group for observing the anti-inflammatory actions of ESM on different inflammatory models. Compared with the blank control group and the positive control group, the high dose group could inhibit ear-swelling of mice induced by dimethylbenzene, edema of rat paw induced by carrageenin and granuloma induced by implanting cotton ball. The high dose of ESM had a significant effect on the inflammation models.