Polymorphic microsatellite markers were developed from black rockfish(Sebastes schlegelii) with an enriched partial genomic library by magnetic beads and the polymorphisms of these loci were assessed in 32 individuals from a wild population. We screened 58 positive clones by three-primers PCR method and sequenced them, then 47 sequences were detected containing microsatellites, and 15 pairs of polymorphic microsatellite primers were obtained. A total of 66 alleles were detected, with the number of alleles ranging from 2 to 14 (averaging 4.4). The observed and expected heterozygosity values ranged from 0.062 5 to 0.968 8 and 0.061 5 to 0.929 1, respectively. The values of polymorphism information content indicated, six loci were highly or moderately polymorphic. HJ2-32,HJ4-27,HJ4-45 and HJ4-47 significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found. The result demonstrates these markers will facilitate relevant research in S. schlegelii.