This study was designed to measure the changes of the carotenoid content in tissues(skin, fin, muscle, eyeball, gill)of pink, red spots and black spots red tilapia, and thus to infer carotenoid expression and sedimentary method between different tissues by adding different amounts of astaxanthin in the feed. The results show that when the dosage of dietary astaxanthin is 400 mg/kg,the carotenoid content in tissues of red tilapia was significantly increased and the body color of red tilapia was significantly improved compared with the control group, while the carotenoid sedimentary amount in the gill of dark spots red tilapia has improved significantly compared to the control group (P<0.05). When the dosage of dietary astaxanthin is 800 mg/kg,the carotenoid sedimentary amount in the fin of pink red tilapia and the gill of dark spots red tilapia has improved significantly compared to the control group (P<0.05),but the other tissues of the carotenoid content of other tissues showed a downward trend compared to the control group and the dosage of 400 mg/kg, and the body color of the red tilapia did not change significantly. Astaxanthin content has a significant impact on the total carotenoid content of red tilapia tissues (P<0.01). The impact of the body color of red tilapia on total carotenoid content was not significant (P>0.05). The interaction between astaxanthin and body color has a more significant effect on carotenoid content (P<0.05). Cluster analysis showed that deposition rate in fin is the highest, followed by gill, and the next is the eyeball, deposition rate in skin and muscle is the lowest.