In the present study, Chinese medicine herbs were formulated and suplemented in diets to feed Litopenaeus vannamei to evaluate the effects on growth, digestive protease activities, hemolymph nonspecific immunity, and muscle composition of shrimp. Six diets were prepared, including basal diet, basal diet added with 1.0% ARR(1/3 Leguminosae, 1/3 Radix scutellariae,1/3 Rheum palmatum L.), 2.0% ARR, 2.0% EARR (1/2 Eucommia ulmoides + 1/2 ARR), 2.0% compound A (including Leguminosae, Epimedium sagittatum, Radix scutellariae, Rheum palmatum L., etc.) and 2.0% compound B (including Leguminosae, Eucommia ulmoides, Honeysuckle flower, Atractylodes macrocephala, etc.). Shrimps with average body weight of (7.5±0.2) g were fed the six diets for 42 days. Results showed that all groups presented high survival rates(above 96.3%)and there were no significant differences among groups(P<0.05). Shrimp fed diet with addition of 2.0% EAAR (145.3%, 1.25), 1.0%AAR (143.8%, 1.26) had significantly higher weight gain and significantly lower FCR than shrimp fed control diet (124.5%, 1.46),P<0.05. Compared with control group, hemolymph non-specific immunity indexes were improved by the addition of herbs at varying degree, and 2.0% EARR group had significantly higher activities of hemolymph ALP and PO than those of control group. The death rate of shrimp at 96 h after being challenged by Vibrio alginolyticus decreased by the addition of 1.0%ARR(P<0.05) in diet compared with control group. Shrimp of 2.0% EARR group had the highest muscle collagen content(0.30%), and was 42.86% higher than that of control group (P<0.05). Results above showed that the addition of 2.0% EARR and 1.0% ARR in diet of white shrimp could improve WGR, reduce FCR, and the addition of 1.0% ARR could enhance resistance against infection with Vibrio alginolyticus, and the addition of 2.0% EARR could significantly improve muscle collagen content.