In this study, observation on molting and growth of Chinese mitten crab (Eriocheir sinensis) at adult stage (200 days) was carried out under laboratory conditions. The results showed that the average growth rate of body weight at the first to fourth molting stages was 48.7%, 69.1%, 80.1%, 64.8%, respectively; the average carapace length growth rate was 21.3%, 17.0%, 29.5%, 16.7%, respectively; the average carapace width growth rate was 17.4%, 18.3%, 28.2%, 18.1%, respectively. The body weight, carapace length and carapace width of male individuals were higher than those of female individuals at each molting stage, and the significant differences (P<0.05) were found since the third molting time. The average molting period was 37 days, 38 days, 29 days and 41 days for the first to fourth molting stages, respectively. The coefficient of variation of growth was higher at the first two molting stages than that at the latter two molting stages. The initial weight had significant effect (P<0.05) on the weight gained after each molting. There was significant difference (P<0.01) in weight,carapace length and carapace width among each molting stage, and the body weight after the third molting stage had significant correlation (P<0.05) with the weight at other molting stages. This result also showed the carapace length and carapace width had significant correlation(P<0.05) with the culturing temperature, however, the body weight had slightly significant difference (r=0.917-0.923, 0.05<P<0.10) with the culturing temperature. Generally, the present study showed that the growth rate was the highest at the third molting stage, indicating its important effect on the growth and culture practice at this stage. Meanwhile, the results showed that the larger size of juveniles, the larger size of adults would be.