Female honeycomb grouper, Epinephelus merra, was selected as research object in this study. Artificial hormones HCG and LHRHa were used for the description and analysis of gonad developmental changes and sexual steroids fluctuation following hormone injection. Purpose of this study is to establish reliable fundamental information for artificial reproduction of honeycomb grouper, and offer significant reference on manipulation practice. According to the results, both the hormones HCG and LHRHa significantly induced spawning and the releasing of MIS, which determines success of ovulation; Estradiol level of honeycomb grouper decreased significantly after spawning identifying the lunar related periodicity of the ovary development in honeycomb grouper species; In fishery productive practice, it is very important to confirm the ovary developmental stage before hormone injection in order to enhance success of inducing maturation, especially for LHRHa injection of which the effectiveness of inducing maturation was the best; As for honeycomb grouper with most oocytes that were in early stage of development, effectiveness of HCG was higher than that of LHRHa, but it was still unsatisfying. Considering the best effectiveness for the ovaries which completed vitellogenesis in LHRHa treatment group and its other advantages over HCG, we highly recommend the application of LHRHa in honeybomb grouper reproductive industry.