The health assessment, evaluation methodology and normal range value of the lake bluntnose black bream (Megalobrama amblycephala) from anatomy, body index, hepatic morphology and color, hepatic index and fat content, section of liver tissue and serum enzyme were studied. The results showed: the normal range value of condition factor, weight/length and viscera index are 2.05-2.33. 14.37-16.51 and 6.36-9.14. The lake bluntnose black bream’s health assessment of body surface color can be evaluated from three respects（1） Color: the color of normal liver is mauve, while dark yellow after being drawn from the caudal vein. （2）External appearance: the edge of liver is clear, linear. And there is no hepatomegaly. （3）Texture: the texture of normal liver is elastic and nonpitting when pressed. The section of liver tissue can evaluate from three aspects: （1）Cell boundaries are clear. （2）Nucleus is located in the center of cells. （3）There is a small quantity of liqid drops in hepatic cell. All of the fifteen serum enzymes are normal distribution or lognormal distribution. And the normal values are: ALT 7.02-21.52 U/L，AST 125.99-361.09 U/L，AST/ALT 10.43-28.87，CHE 65.97-213.95 U/L，ALP 12.07-41.81 U/L，TP 13.60-23.36 g/L，AlG 9.76-13.78 g/L，Glo 3.99-9.44 g/L，STB 2.17-4.02 μmol/L，TBA 2.08-22.46 μmol/L，Glu 2.81-7.36 mmol/L，CHO 4.77-8.99 mmol/L，TRIG 0.27-0.72 mmol/L，HDL C 2.18-4.75，LDL C 1.71-4.77 mmol/L，Cr 0.95-4.56 μmol/L.