The experiment was conducted to study effect of three kinds of feed on growth, immune enzyme, amino acid content and digestive enzymes activity of Procambarus clarkii for two months. The results showed that the Procambarus clarkii’s SOD,CAT activity of biological feed was higher than other groups(P<0.05)，while ACP，LSZ have no significant difference (P>0.05). Three feed has little influence on the composition and content of amino acid in Procambarus clarkii. The composition and content of essential amino acid and the ratio of the total amino acid are not due to the different feed. In addition, biological feed has a little influence on the growth of Procambarus clarkii, the weight gain rate was higher than the other feed’s. At the same time, trypsin and amylase activity were higher than ordinary feed’s, and trypsin activity was significantly different from ordinary feed’s (P<0.05). Therefore biological feed can enhance the Procambarus clarkii’s immunity, improve the viability and accelerate its growth.