According to zebrafish GDF9 and BMP15 gene conservative region of nucleotide sequence to design primers, the GDF9 and BMP15 gene fragment of Carassius curatus cuvieri was amplified by RT PCR. The partial sequence of GDF9 is 778 bp and codes a protein of about 145 amino acids, the partial sequence of BMP15 is 811 bp and codes a protein of about 270 amino acids. The amino acid sequence comparison results showed that the GDF9 gene of Carassius curatus cuvieri shared 58%-78% identity with other species,and Carassius curatus cuvieri is most similar to zebrafish（78%）; BMP15 gene ofCarassius curatus cuvieri shared 30%-80% identity with other species,and it is most similar to zebrafish（80%）.Phylogenetic tree of GDF9 and BMP15 gene generated by Neighbor Joining method suggested that all teleosts cluster together，and Carassius curatus cuvieri GDF9 is clustered closely with that of zebrafish, rainbow trout and European sea bass, and Carassius curatus cuvieri BMP15 is clustered closely with that of zebrafish and European sea bass.Then the expressions of different tissues including spleen, gill, liver, brain, heart, muscle, kidney and ovary were analyzed by semi quantitative PCR.The results showed that the BMP15 gene of Carassius curatus cuvieri was expressed in all tissues and highest in ovary; the GDF9 gene of Carassius curatus cuvieri was expressed in liver, brain, heart, muscle and ovary, while highest in ovary and extremely low even no expression in spleen, gill and kidney. This study will be helpful for further understanding the structure and function of GDF9 and BMP15 gene.