To further understand the ossificational process of intermuscular bones in fishes from Cyprinidae, an important farming fish Hemibarbus labeo was investiaged in this study. Intermuscular bones of H. labeo at different developmental stages were stained with alizarin red. Before 35 dpf (days post fertilization), all bones in H. labeo were ossified except for intermusclar bones. Since 35 dpf, intermuscular bones in tail area begun ossification in 23.67 cm standard length H. labeo. The ossified epineurals were observed in the myoseptums from 37thto 41th myotome, and the ossified epipleurals was only found between 39th to 40th myotome. As H. labeo was developping, ossified intermuscular bones were gradually observed in the myoseptums close to the head. Till to 62 dpf, all intermuscular bones were found ossified in H. labeo with 30.03 cm standard length. In addition, the primary morphology of intermuscular bone was type I, and ossified intermuscular bones with more complicated morphology were gradually forming from the type I during ossifing process. On the whole, the timing of intermucular bone initial ossification and the forming process of complex morphology in H. labeo were very similar to that in other species in Cyprinidae, indicating conserved genetic mechanism control of ossification of intermuscular bones might exist in Cyprinidae.