A set of 8 microsatellite markers were used for parentage identification in obverse cross group of Heilongjiang carp♀×German mirror carp♂, and inverse cross group of German mirror carp♀×Heilongjiang carp♂. The results showed that: (1) observed heterozygosity values of parents and offspring were 0.607 1, 0.714 3, 0.681 8 and 0.708 3, 0.538 6, 0.743 8, and polymorphism information content were 0.442 1, 0.281 3, 0.553 5 and 0.673 5, 0.375 9, 0.624 1 in two groups, indicating that all 8 microsatellite loci showed polymorphism. (2) In the obverse cross group, the accumulative exclusion rate using 8 microsatellite loci was 99.92% and the appraisal ability was 98.00% while confidence was 95%. In the inverse cross group, the accumulative exclusion rate was 99.99% and the appraisal ability was 99.00%. (3) about relation between microsatellite markers and appraisal ability, we compared the accumulative exclusion rates and the appraisal ability using 3, 4, 5, 6, 7, 8 microsatellite markers. 6 and 8 high polymorphism markers were successfully applied in paternity determination of two cross groups.