In present research work, the changes of the biochemical composition and energy density in hepatopancreas and muscle of Procambarus clarkii before and after spawning and during the berried stages were studied. The results showed that (1) before and after spawning stages there was no significant change for indices of hepatopancreas and muscle (P>0.05), while the ovarian index decreased from 6.14% to 0.40% (P<0.01). Meanwhile, the hepatopancreas index increased by 13.29% from after spawning stage to berried stage , which was significantly higher than that before spawning stage; (2) The crude protein, crude lipid and carbohydrate decreased significantly after spawning, in which crude lipid in hepatopancreas and crude protein in muscle decreased significantly (P<0.01). During the berried stage, the protein and carbohydrate were quickly recovered and exceeded the levels of those before spawning stage (P<0.01). The energy in hepatopancreas decreased significantly before and after spawning stage,while the energy in muscle decreased significantly at the berried stage; (3) The PUFA was decreased significantly after spawning stage, in which DHA and EPA decreased by 44.33% and 30.80% respectively. During the three stages , the C20∶4n-6 displayed an increasing pattern, while the SFA and MUFA were stable, and only increased slightly.