Hydantoin compounds were a kind of fivemembered heterocyclic compounds,and its derivatives could be used as important intermediate of pharmaceutical products, pesticides, disinfectant, photographic materials. The development of hydantoin compounds had great significance. In this paper, a series of new hydantoin compounds were synthesized by 3-bromo phenylacetic acid as starting material, 7-bromo-2-tetralone as intermediate and with Ullmann reaction, Suzuki reaction and BuchererBegs reaction as key steps. The structures of key intermediate and target compounds were confirmed by ¹H NMR and ¹³C NMR. Bioactivity of target compounds was tested by the mode of oral administration and then using ammonia water induced cough model, respiratory tract of the mice was stimulated by ammonia with ultrasound nebuliser, and record the cough incubation period of mice and the cough times within two minutes. Bioactivity research showed that the target compounds could reduce ammonia spray frequency cough in mice, extend the cough incubation period, had significant antitussive effect on mice of ammonia-induced cough, and that could lay the basis for hydantoin compounds application development.