Apparent digestibility coefficients (ADC) of three selected feed ingredients fishmeal, soybean meal and corn gluten meal of Takifugu obscurus were measured. A basal diet was formulated to contain 45.52% crude protein, and three kinds of test diets were formulated by combining the basal diet and each of the tested ingredients at a ratio of 70∶30.Chromicoxide was added at 0.5% in the basal diet and test diets as inertmarker. A basal diet was formulated to contain 45.40% crude protein, two experiments diets were prepared by adding 0.050% and 0.075 % multi-enzyme, respectively, the apparent digestibility of above three diets were measured. The results showed that the protein apparent digestibility of fish meal, soybean meal and corn gluten meal were higher, 93.40%，91.83% and 94.97%,respectively. Feed dry matter apparent digestibility of fish meal, soybean meal and corn gluten were 88.13%, 85.73% and 89.25%, respectively. Multi-enzyme supplemented could significantly improve the apparent digestibility of protein and dry matter of Takifugu obscurus. However, apparent digestibility has no significant difference between the 0.050% and 0.075% experimental groups.