The growth relationship between statolith and individual of the jumbo flying squid (Dosidicus gigas) sampled in waters off Costa Rica from July to August in 2009 was studied with multiple statistical methods. It was found that the growth relationship between statolith and mantle length (ML) was significant (P<0.01), and the biggest coefficient were ventral dorsal dome length (DDL) and total statolith length (TSL). Statolith growth contained two phases, rapid growth of statolith before ML was smaller than 26-30 cm, and after that there has a slow growth of statolith. The dorsal length (DL), rostrum inside length (RIL) and DDL grew faster than the TSL, but the ventral dorsal dome angle (DDA), rostrum lateral dome angle (RDA) and rostrum angle (RA) were reduced during the growth process. So the morphology of lateral dome and rostrum were changed significantly (P<0.05). Although the significant changes of statolith morphology preceded the growth changes and the significant changes of various parts in morphology were not synchronized, the morphological changes mainly occurred in individuals with 23-29 cm ML, after that the morphology kept stabilized. The significant changes of growth and morphology were associated with habitat changed of water layer, and the jumbo flying squid statolith was coincided with the characteristics of pelagic cephalopods.