This paper investigated the trypsinase, acid protease, amylase and lipase activities of the Oplegnathus fasciatus larvae cultured in indoor pond from hatching to post hatching day (phd) 33^rd. The results indicated that trypsinase, acid protease, amylase and lipase could be detected in newly hatched larvae, although the activities of trypsinase, acid protease and amylase were very low. The total activity of trypsinase (U/larva) of the larvae increased significantly on phd 9^th and increased gradually with the development. The total activities of acid prolease and lipase of the larvae increased significantly on phd 28^th. The total activities of acid prolease and lipase of the larvae on phd 33^rd were significant higher than those on phd 28^th respectively. The total activity of amylase of the larvae increased significantly on phd 9^th and also increased gradually with the development. While the larvae developed at juvenile stage (>phd 22^nd), the total activity of amylase decreased significantly with the development. It is suggested that the activities of digestive enzyme of the Oplegnathus fasciatus larvae closely correspond to the development stage and feeding habit. The trypsinase and amylase were the very important endogenous enzymes at the early stage of the larva development.