This study was conducted to evaluate the effect of lipase enzyme preparation additive to Pseudobagru vachelli diet on growth performance,digestive enzyme and serum biochemical indexes.P.vachelli with the average initial weight of(3.47 ± 0.05) g were randomly divided into four groups and fed on diets added with 0,100 mg /kg,300 mg /kg and 500 mg /kg lipase for 90 days.The test results showed that: growth rate of 100 mg /kg,300 mg /kg and 500 mg /kg lipase additive groups was increased by 3.86% (P>0.05), 9.29% (P<0.05), 6.75% (P>0.05), and the coefficient of feed decreased by 3.17% (P>0.05), 6.35% (P<0.05), 5.29% (P<0.05). Compared with control group,the addition of lipase significantly lowered the contents of triglyceride and total cholesterol,low-density lipoprotein cholesterol in blood(P<0.05). The lipase activity of midintestine and trypsin activity were significantly higher than those of the control (P<0.05). Meanwhile,the lipase and amylase activities of hepatopancreas significantly improved in the group with addition of 100 mg /kg lipase(P<0.05). The lipase activity of hepatopancreas was highest with 300 mg /kg lipase additive.In conlusion,it showed that the addition of about 300 mg /kg might be helpful for improving the growth performance of P.vachelli.